Alex de Minaur will play his Wimbledon first-round match against in-form Australian compatriot James Duckworth, while Ajla Tomljanović will face 13th seed Jeļena Ostapenko.
Ninth seed de Minaur had initially been drawn to play Corentin Moutet, but the Frenchman withdrew from the tournament because of a wrist injury.
This offered up a dream opportunity for Duckworth — who had been knocked out in the final round of qualifying — to play de Minaur as a 'lucky loser'.
World number 55 Moutet had promised to be an unpredictable opponent for de Minaur.
But Duckworth, the world number 78, poses just as much danger to ninth-ranked de Minaur, having recently reached the Stuttgart quarterfinals on grass.
De Minaur is still favourite to win through and meet 17th seed Felix Auger-Aliassime in the last 32, should the Canadian get past another Australian in Thanasi Kokkinakis in the first round.
A potential titanic fourth-round date with Hubert Hurkacz would also have to be negotiated for de Minaur before a potential match-up with seven-time champion Novak Djokovic.
Djokovic, seeded second, is set to play just weeks after undergoing knee surgery. His first-round opponent will be Czech qualifier Vít Kopřiva.
Australia's Max Purcell, who has made his first ATP Tour final at the grass-court Eastbourne International, plays Finland's Otto Virtanen in the first round.
With Duckworth's late inclusion, 11 Australian men feature in the singles draw.
Qualifier Alex Bolt landed the most difficult assignment against Norway's eighth seed Casper Ruud as reward for battling through an amazing qualifying week.
In the women's singles draw, Australian wildcard Tomljanović will contest Wimbledon fresh from reaching the Birmingham Classic final.
She will play 2017 French Open winner Ostapenko in the first round, three years after they clashed in an ill-tempered fourth-round contest at Wimbledon.
Tomljanović won that match in three sets, but Ostapenko labelled Tomljanović the "worst player on the tour" after she had been accused of faking injury.
The match ended with Ostapenko confronting Tomljanovic at the net, saying: "Your behaviour is terrible, terrible. You have zero respect."
But Tomljanović is adamant there was no bad blood between the pair.
Tomljanović played Ostapenko in Eastbourne in 2022 and at this year's Australian Open, losing on both occasions.
"We're cool, I would say," said Tomljanovic.
"Look, for me, what happened is in the past."
Australian number one Daria Saville faces American Peyton Stearns, while Olivia Gadecki will play in a battle of the qualifiers against US teenager Robin Montgomery.
AAP