Novak Djokovic's hamstring injury scare continues to hover over his Australian Open tilt after the nine-time champion required treatment on his left leg throughout an exhibition practice match against Daniil Medvedev.

Djokovic pushed through hamstring tightness to claim last week's Adelaide International 1 and firm as favourite for a 10th Melbourne Park title.

He tweaked his left hamstring in the semifinal in South Australia when also playing Medvedev and required a medical timeout, but he recovered to win that game, then the final.

Wednesday's practice match with Medvedev at Rod Laver Arena was the first time Djokovic had played in front of the Melbourne public since last year's deportation saga.

The session was meant to be 75 minutes but Djokovic exited after Medvedev claimed the first set 6-4 in 36 minutes.

He broke the Russian former world No.1 in the opening game but, when leading 3-2, received treatment, with a member of his team completing some knee extensions.

Knee extensions can be used to release the hamstring.

He only won one more game from that point.

Medvedev won the next two games and Djokovic again received the same treatment at the break when trailing 4-3 and again at 5-4, before pulling the pin when the Russian triumphed a game later.